Cloning of a Novel Phosphatidylinositol Kinase-related Kinase

NOVEL REAGENTS FOR CREATINE KINASE

The purpose of this investigation was to develop a simple colorimetric method for creatine kinase (CK). The new method is based on the reaction of creatine, formed enzymatically from creatine phosphate and ADP, with different glyoxal compounds. Hydrated glyoxals, such as para-nitrophenyl, 2-thiophene, 4- biphenyl, 4, 4' -biphenyl,α-naphthyl, β-naphthyl, para-chlorophenyl, and styryl were...

Phosphatidylinositol 3-kinase: a novel effector.

A number of years ago, when the raison d’#{234}tre for tyrosine kinase activity was completely unclear, one of the tantalizing leads was the apparent coincidence of inositol lipid kinase activity with certain tyrosine kinases (see, for example Refs. 1 and 2); the exciting prospect implied was a link to a well defined signal transduction pathway (for a review of inositol lipid kinases, see Ref. ...

novel reagents for creatine kinase

the purpose of this investigation was to develop a simple colorimetric method for creatine kinase (ck). the new method is based on the reaction of creatine, formed enzymatically from creatine phosphate and adp, with different glyoxal compounds. hydrated glyoxals, such as para-nitrophenyl, 2-thiophene, 4- biphenyl, 4, 4' -biphenyl,α-naphthyl, β-naphthyl, para-chlorophenyl, and styryl were s...

Inhibition of lymphocyte kinase Lck and phosphatidylinositol 3-kinase by a novel immunosuppressant, lymphostin.

Lck is a Src-family tyrosine kinase that is expressed predominantly in T cells, where it plays important roles in T-cell activation. Lymphostin was isolated from Streptomyces sp. as an inhibitor of Lck. As previously reported, lymphostin inhibited Lck (IC50 0.05 microM) and the mixed lymphocyte reaction (IC50 0.009 microM). We have now examined the mechanism of inhibition by lymphostin. Lymphos...

Involvement of Phosphatidylinositol 3-Kinase